See TB-500 dosage trends, reconstitution patterns, and vial size preferences from anonymized WPA peptide calculator data.
TB-500 (thymosin beta-4 fragment) is widely researched for its role in wound healing, tissue repair, and inflammation modulation. This data reveals the dose amounts, vial sizes, and bacteriostatic water volumes researchers most commonly choose when setting up their TB-500 reconstitution protocol.
TB-500 is a synthetic 17-amino-acid fragment of thymosin beta-4 — a small, naturally occurring G-actin-sequestering peptide originally isolated from calf thymus and characterized through the Goldstein et al. body of work across the 1980s and 1990s. Thymosin beta-4 itself is one of the most abundant intracellular peptides in mammalian tissue, and the downstream literature established it as a regulator of cell migration, wound repair, angiogenesis, and inflammation: it accelerates corneal re-epithelialization in animal models, supports cardiac repair after experimental infarction, and increases dermal-wound closure speed in rodents and pigs.
TB-500 is one of the most-sourced research peptides for soft-tissue and recovery protocols, often stacked with BPC-157. It ships as a lyophilized powder you reconstitute with bacteriostatic water before use — but note that "TB-500" (a 17-amino-acid fragment) is frequently conflated with full-length thymosin beta-4.
The Tβ4 N-terminus binds monomeric G-actin to regulate actin polymerization, and the same domain promotes endothelial-cell migration, capillary formation, and hematopoietic stem-cell mobilization. TB-500 is hypothesized to retain the actin-binding and pro-angiogenic activity of the parent molecule.
RegeneRx and collaborators have studied full-length Tβ4 in dermal wound healing (Phase 2 venous-stasis ulcers), dry-eye disease (Phase 3 RGN-259), post-myocardial-infarction cardiac repair, and corneal injury. The fragment "TB-500" itself has been characterized largely in animal tendon and cardiac models. WADA has prohibited TB-500 in competitive sport since 2011.
TB-500 has a reported half-life of ~2 days, and the dose intervals researchers model most often fall around twice weekly. Twice-weekly dosing reaches steady state in about 2 weeks. The trough level between doses sits at roughly half of the peak, so levels stay fairly stable across the week. It is catalogued under Recovery Peptides on WPA.
3,493 TB-500 reconstitution calculations have been logged by the WPA community. The most common dose entered is 2mg (892 calculations). The most common bacteriostatic water volume is 2mL. The most popular vial size is 10mg (2,193 sessions). The most common dosing frequency is once weekly (335 logged protocols), followed by 2x/week (328).
These figures come from anonymized WPA peptide calculator sessions.
All figures shown are aggregated from anonymized calculator inputs and are provided strictly for independent laboratory research and educational purposes. They are community usage statistics — not dosing recommendations, and not medical advice.