Explore PNC-27 dosage trends, reconstitution volumes, and vial size patterns from anonymized WPA peptide calculator sessions.
PNC-27 is a synthetic peptide combining a p53-derived sequence with a membrane-penetrating leader domain, studied in preclinical cancer research for its proposed ability to selectively disrupt the membranes of cancer cells that express HDM-2 on their surface while sparing normal cells. This data shows the dose amounts, vial sizes, and bacteriostatic water volumes researchers most commonly select when reconstituting PNC-27.
PNC-27 is a 32-residue chimeric peptide studied in preclinical cancer-cell research for selective membrane lysis. It is a research-only molecule that ships as a lyophilized powder, so vendor quality comes down to what the certificate of analysis (COA) shows.
PNC-27 binds HDM-2 (the human homolog of MDM2) on the surface membrane of cancer cells — where HDM-2 is anomalously expressed compared to non-transformed cells. The peptide–HDM-2 complex inserts into the plasma membrane and forms transmembrane pores, leading to rapid colloid-osmotic necrosis of the cancer cell. Healthy cells do not display membrane HDM-2 and are reportedly spared. This is a necrosis-via-membrane-disruption mechanism, distinct from the apoptosis-induction mechanism of most p53-pathway therapeutics.
Michl, Bowne, and colleagues have published a series of pre-clinical papers reporting PNC-27 selectivity for cancer-cell lines (pancreatic, breast, leukemia, glioma) over non-transformed cells in vitro, and tumour-growth suppression in mouse xenograft models. No independent group has produced a major replication of the membrane-HDM-2 selectivity claim, and no human clinical trial has been registered.
PNC-27 has a reported half-life of ~30 min (pre-clinical estimate), and the dose intervals researchers model most often fall around once daily. This p53-derived membrane-disrupting peptide clears from plasma quickly; there is no systemic buildup, and its action is driven by local exposure rather than a steady blood level. It is catalogued under Other Peptides on WPA.
8 PNC-27 reconstitution calculations have been logged by the WPA community. The most common dose entered is 100mcg (3 calculations). The median dose across all sessions is 200mcg. The most common bacteriostatic water volume is 3mL. The most popular vial size is 10mg (4 sessions).
These figures come from anonymized WPA peptide calculator sessions.
All figures shown are aggregated from anonymized calculator inputs and are provided strictly for independent laboratory research and educational purposes. They are community usage statistics — not dosing recommendations, and not medical advice.