Explore PE-22-28 dosage trends, reconstitution volumes, and vial size patterns from anonymized WPA peptide calculator data.
PE-22-28 is a short spadin-derived peptide studied as a TREK-1 channel blocker in antidepressant research. It ships as a lyophilized powder you reconstitute before use, so the real vendor difference is what the certificate of analysis (COA) shows.
PE-22-28 selectively blocks the TREK-1 (KCNK2) two-pore-domain potassium channel — the same channel pharmacologically targeted by fluoxetine and inferred as a candidate antidepressant mechanism from TREK-1-knockout mouse studies. By antagonising TREK-1, PE-22-28 increases serotonergic neuron firing in the dorsal raphe and produces rapid antidepressant-like behavioural effects in rodent models.
Pre-clinical rodent work has reported antidepressant-like effects in forced-swim, tail-suspension, and chronic-mild-stress paradigms with onset faster than classical SSRIs, and Borsotto group follow-up has explored stroke neuroprotection and post-stroke depression. No registered human clinical trial has been conducted.
PE-22-28 has a reported half-life of ~few hours (estimated), and the dose intervals researchers model most often fall around once daily. This spadin-derived TREK-1 blocker was engineered to be more stable than spadin (whose half-life is only minutes), but plasma exposure is still short, so it acts largely per-dose. It is catalogued under Memory Peptides on WPA.
47 PE-22-28 reconstitution calculations have been logged by the WPA community. The most common dose entered is 50mcg (18 calculations). The median dose across all sessions is 150mcg. The most common bacteriostatic water volume is 2mL. The most popular vial size is 10mg (46 sessions). The most common dosing frequency is daily (7x/week) (2 logged protocols), followed by 2x/week (1).
These figures come from anonymized WPA peptide calculator sessions.
All figures shown are aggregated from anonymized calculator inputs and are provided strictly for independent laboratory research and educational purposes. They are community usage statistics — not dosing recommendations, and not medical advice.