Explore Humanin dosage trends, reconstitution volumes, and vial size patterns from anonymized WPA peptide calculator sessions.
Humanin is a 24-amino-acid mitochondrial-derived peptide encoded within the mitochondrial 16S rRNA gene, studied for its cytoprotective and anti-apoptotic effects and its proposed roles in metabolic regulation, neuroprotection, and longevity. It is frequently researched alongside MOTS-c as part of the mitochondrial-derived peptide family. This data shows the dose amounts, vial sizes, and bacteriostatic water volumes researchers most commonly select when reconstituting Humanin.
Humanin is a 24-residue mitochondrial-derived peptide studied for cytoprotection and metabolic-aging research. It ships as a lyophilized powder you reconstitute before use, so vendor quality comes down to what the certificate of analysis (COA) shows.
Humanin binds intracellularly to pro-apoptotic Bcl-2 family proteins (Bax, Bid, BimEL), blocking their mitochondrial pore-forming activity and preventing cytochrome-c release. Extracellularly, it signals through a heterotrimeric receptor complex (CNTFR/WSX-1/gp130) on the cell surface to activate STAT3, and at higher concentrations also engages the formyl-peptide receptor 2 (FPR2/ALX). These two signalling arms — intracellular anti-apoptotic and extracellular cytoprotective — underlie the published pre-clinical findings.
Hashimoto 2001 established the anti-Alzheimer-β-toxicity signal that named the peptide. Muzumdar 2009 showed humanin and its analogue HNG improve insulin sensitivity in rodents. Lee 2015 (Cohen group) reported that circulating humanin levels decline with age in humans and rhesus macaques, and that low levels correlate with cognitive decline and metabolic disease risk. No registered human clinical trial of humanin or its analogues has reported efficacy data to date.
Humanin has a reported half-life of ~1 hour, and the dose intervals researchers model most often fall around once daily. Plasma Humanin clears within hours; there is no meaningful systemic buildup between daily doses. Downstream mitochondrial signalling is thought to outlast the plasma half-life. It is catalogued under Anti-Aging Peptides on WPA.
21 Humanin reconstitution calculations have been logged by the WPA community. The most common dose entered is 500mcg (7 calculations). The median dose across all sessions is 750mcg. The most common bacteriostatic water volume is 2mL. The most popular vial size is 10mg (17 sessions). The most common dosing frequency is once weekly (1 logged protocols), followed by 2x/week (1).
These figures come from anonymized WPA peptide calculator sessions.
All figures shown are aggregated from anonymized calculator inputs and are provided strictly for independent laboratory research and educational purposes. They are community usage statistics — not dosing recommendations, and not medical advice.